Mesenchymal stem cells overexpressing Ihh promote bone repair

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Mesenchymal stem cells overexpressing Ihh promote bone repair

BACKGROUND Indian hedgehog (Ihh) signaling pathway is known to play key roles in various aspects of normal endochondral bone development. This study tested the potential roles of high Ihh signaling in the context of injury-induced bone regeneration. METHODS A rabbit tibia defect model was established to test the effects of the implant of Ihh/mesenchymal stem cells (MSCs)/scaffold complex. Com...

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Bone marrow-derived mesenchymal stem cells overexpressing miR-21 efficiently repair myocardial damage in rats

OBJECTIVE We investigated the ability of bone marrow derived mesenchymal stem cells (BMSCs) overexpressing microRNA-21 (miR-21) to repair cardiac damage induced by anthracyclines in rats. METHODS Sprague-Dawley (SD) rats of 2~3 weeks old were selected to isolate and culture BMSCs. A lentivirus harboring pLVX-miR-21 was generated and transfected into rat BMSCs. The rats were assigned into an u...

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Bone marrow mesenchymal stem cells promote the repair of islets from diabetic mice through paracrine actions.

Transplantation of bone marrow mesenchymal stem cells (MSCs) has been shown to effectively lower blood glucose levels in diabetic individuals, but the mechanism has not been adequately explained. We hypothesized that MSCs exert beneficial paracrine actions on the injured islets by releasing biologically active factors. To prove our hypothesis, we tested the cytoprotective effect of conditioned ...

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Cellular Biology IGF-1–Overexpressing Mesenchymal Stem Cells Accelerate Bone Marrow Stem Cell Mobilization via Paracrine Activation of SDF-1 /CXCR4 Signaling to Promote Myocardial Repair

We hypothesized that mesenchymal stem cells (MSCs) overexpressing insulin-like growth factor (IGF)-1 showed improved survival and engraftment in the infarcted heart and promoted stem cell recruitment through paracrine release of stromal cell–derived factor (SDF)-1 . Rat bone marrow–derived MSCs were used as nontransduced (MSCs) or transduced with adenoviral-null vector (MSCs) or vector encoding...

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Harvesting of bone marrow mesenchymal stem cells from live rats and the in vitro differentiation of bone marrow mesenchymal stem cells into neuron-like cells

In the bone marrow, there are certain populations of stem cell sources with the capacity to differentiate into several different types of cells. Ideally, cell transplants would be readily obtainable, easy to expand and bank, and capable of surviving for sufficient periods of time. Bone marrow mesenchymal stem cells (BM-MSCs) possess all of these characteristics. One of the most important benefi...

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ژورنال

عنوان ژورنال: Journal of Orthopaedic Surgery and Research

سال: 2014

ISSN: 1749-799X

DOI: 10.1186/s13018-014-0102-7